dextran hydrodynamic radius

WebFluorescent and biotinylated dextrans are routinely employed to trace neuronal projections. (1998). The micropores of the human cadaver skin were regenerated according to the time. This method utilizes promising strategies that have made a significant impact in transdermal delivery [6], they include: iontophoresis, microneedles, sonophoresis, radiofrequency (RF), and chemical enhancers [7,8,9,10,11]. Huggins equation [41] gives the relation to the relative viscosity increase (i). Advances in Carbohydrate Analysis, 1, 63-144. Catiker, E.; Guner, A. Bahary, W.S. Challenges in analysis of high-molar mass dextrans: Comparison of HPSEC, AsFlFFF and DOSY NMR spectroscopy. Morris, G.A. Bohdanecky, Miloslav (1996). ; Grishchenko, A.E. Journal of Applied Polymer Science, 13, 2031-2032. Keywords: intrinsic viscosity, dextrans, Mark-Houwink parameters, hydrodynamic, temperature, Journal of Polymer and Biopolymer Physics Chemistry, 2013 1 (1), This is because the macromolecule hydrodynamic radius changes the solution type and temperature via change in their chain exibility. This situation is mainly the result of using dextans, solvent, additives, etc. The micropore formed at the SC through to the epidermis, enabling drug delivery. However, in vitro investigations are very few. Chemical penetration enhancers for transdermal drug delivery systems. Naik A, Kalia YN, Guy RH. Pore closure examination was performed using an optical microscope (BX43, Olympus, Japan) immediately (time 0), 4, 8, and 24h following device application. Curley SA. The (a/b) value is 2.5 which confirms that dextran in aqueous solution is a biopolymer with a spherical conformation for an Mw range from 8.8 to 200kDa, and with tendency to compaction with increasing temperature (RH decreases). 151-166. 2009;301(8):60913. ; Ghosh, T.K. A sugar cane native dextran as an innovative functional excipient for the development of pharmaceutical tablets. Progress in Polymer Science, 30, 38-70. The characteristic Rg/Rh value for a globular protein is ~0.775, which means that Rg is smaller than Rh. 24h after microporation, the pore sizes reduced from 64.8817.20m (0h) to 48.854.27m (24h), and the pore depths reduced from 83.9120.56m (0h) to 63.9527.61m (24h). Format), Citation-(BibTeX Gums and stabilisers for the Food Industry, 5, 447-450. Figure3 shows a magnified image of human cadaver skin with microchannels formed by the microporation device (250kHz) and the skin closure over time. This site needs JavaScript to work properly. The enhanced permeability through micropores was confirmed by Franz diffusion cell. 7 8 SWC designed and coordinated the research. Ioan, C.E. In a poor solvent which shows a positive heat of mixing, segments of a polymer molecule attract each other in solution more strongly than attract the surrounding solvent molecules. Synthesis and characterization of blue dextrans. Biophysical Journal, 27, 177-181. (2013). Dextran Specifications: Label (Ex/Em): Fluorescein (494/521) Size: 40,000 MW. Dextran theta temperature between 43.14 and 44.44C for all the range of molecular weights explains the deviation at 45 and 50C [36, 37]. The lack of data on the uniformity of intrinsic viscosity measurements in the system water/dextran, highlights the significant influence of the solvent and temperature [74]. These results suggest that fibroblasts proliferate and migrate under the SC, and regenerate the skin. Figure1 shows a schematic illustration of transdermal delivery after the application of the microporation device. Privacy 2015;32(5):170413. Amersham Biosciences, AA Edition, www.amershambiosciences.com. 2018 Jan;184(1):1-11. doi: 10.1007/s12010-017-2522-4. Curvale, R.; Masuelli, M.; Perez Padilla, A. Dear Sir. Alan F Rawle , Can you help me in calculating the size of dextran 4 (molecular weight= 4000) and dextran 10 (molecular weight 10,000)? Th Polyelectrolytes as secondary calibration standards for aqueous SEC. FOIA Human cadaver skins (fresh human full-thickness skin disc, 12~20mm) were supplied by Biopredic International (Saint-Grgoire, France). Journal of Applied Polymer Science, 72, 871-876. Kloster, C.; Bica C.; Rochas C.; Samios, D.; Geissler, E. (2000). Biomaterials. Proc of SPIE. where (poise) is the viscosity, the (g/cm3) fluid density, and t the liquid draining time (s) subscript zero is referred to solvent, and without subscript is referred to the solution [40], and inherent viscosity is i = r -1. Journal of Polymer Science, 17, 527-546. Hi Sir Alexander Malm , Thank you very much! Really appreciate your help! Immediately after formation, the microchannels are filled with interstitial fluid, making them hydrophilic, and a suitable drug delivery system [15]. The solution was measured using an Anton Paar DMA35N densitometer. They concluded that a power law behavior seems to be approached only in the small molecular weight region. WebFITC Products. California Privacy Statement, Advanced Drug Delivery Reviews, 60, 1650-1662. Journal of Chemical Education, 80(9), 1036-1038. Chen Rong Huei; Min Lang Tsai (1998). Chromatographic characterization of polymers: Hyphenated and multidimensional techniques. Furthermore, the influence of temperature on the intrinsic viscosity is given by the chain flexibility parameter (dln[]/dT), which gives information about the conformation of the macromolecule chain in solution [62]. Chem. Expert Opin Drug Deliv. The hydrodynamic radius is important in predicting transretinal penetration Carroll PJ. In a good solvent, which shows a zero or negative heat of mixing with the polymer, the molecule is less-extended, and the intrinsic viscosity is high. When applied to adherent cell cultures, the APGD-t is capable of inducing temporary cell permeabilization. (2012). Microporation techniques for enhanced delivery of therapeutic agents. The genus Weissella: taxonomy, ecology and biotechnological potential. Carbohydrate Polymers, 74, 372-378. Levin G, Gershonowitz A, Sacks H, Stern M, Sherman A, Rudaev S, Zivin I, Phillip M. Transdermal delivery of human growth hormone through RF-microchannels. Arond L.H. A novel correlation for protein diffusion coefficients based on molecular weight on radius of gyration. Static and Dynamic Light-Scattering Solution Behavior of Pullulan and Dextran in Comparison. 2012;9(2):14754. Walkenhorst, S.; Olivier, R. (2003). where c is the solute concentration (g/cm3), [] is the intrinsic viscosity (cm3/g), and kH represents Huggins constants (cm3/g). ; Rani, K.Y. DOI: 10.12691/jpbpc-1-1-3, Received September 18, 2013; Revised October 23, 2013; Accepted November 22, 2013. WebThis value is sensible given that i j = 0 and that the search radius of the clustering algorithm is slightly larger than the average segment length of our Dextran model (b 5.5 nm). Viscosity, sedimentation, and light-scattering properties of fraction of an acidhydrolyzed dextran. WebDiffusivity of 2000 kDa Dextran was significantly lower for both hydrogel formulations, but was insensitive to collagen concentration, likely due to a larger hydrodynamic diameter ( dh ~ 70 nm) on order of the collagen mesh size. Markiertes Dextran wird hufig per Mikroinjektion in die Zellen eingebracht. The value of qM-H varies from one sample to another one, because it is a function of a and the average molecular weights (Mv, Mw). The Viscosity of Dilute Solutions of Long-Chain Molecules. Bahary, W. S.; Hogan, M. P.; Jilani, M.; Aronson, M. P. (1995). It is well tolerated on parenteral infusion by most people but a very low incidence The same way, many of these authors emphasize the non-linear or hyperbranched of dextran molecular weight range from 9 to 2000kDa [3,5,8,16, 37,38]. Viscosity of Liquids. WebDextran produced from Weissella cibaria JAG8 was purified and characterized. The value of kM-H in this work decreases with increasing temperature in contrast to published by reference [34] where kM-H increases. WebDextran 670 GPC Standard Normative Mw - 670000 Da GPC standards can be used for calibration and molecular weight determination. Granath, K. A. Journal of American Chemical Society, 64(11), 2716-2718. 2013, Article ID 360239, pp. Differences were considered statistically significant at p <0.05. In the case of the work performed in references [34, 35, 36] they studied using the number average molecular weight, which rearranges the value of a and forces it to enter into unbranched or linear molecules, on the other hand, if molecular weights are used the value would be less than 0.5. WebTo address this perception, in the current study, FITC-dextran of different MWs (10, 70 and 150 kDa) was used to model the transdermal delivery of low MW biotherapeutics and mAbs with MWs of approximately 150 kDa. Dependence on Concentration. At 25C both values of a and kM-H are within the range reported by [3, 8, 16]. Physicochemical studies of oligodextran. The most widely used method for the characterization of macromolecules is the capillary viscometry, as it is a simple and economic method. Expert Rev Med Devices. Biomacromolecules 8, 2464-2475. Determination of intrinsic viscosities of macromolecules and nanoparticles. 2008;28(5):64557. Pharm Res. Kasaai, Mohammad R. (2007). Citation-(RIS Rissmann R, Oudshoorn MHM, Hennink WE, Ponec M, Bouwstra JA. To improve drug permeability, micropore ablation using RF is performed by passing an alternating electrical current through the skin at a frequency higher than 100kHz. 20K. * shows a significant difference between the M.W. Sintov AC, Krymberk I, Daniel D, Hannan T, Sohn Z, Levin G. Radiofrequency-driven skin microchanneling as a new way for electrically assisted transdermal delivery of hydrophilic drugs. Dextran consists of -D glucose units with a majority of (16) glucosidic linkages between them. GPC Symp. The human skins were from the back or thigh of a 24-year-old woman. Gekko, K. (1971). The range of temperatures used was 25-50 (0.1) C and it was regulated by thermostatic bath Haake C. The viscosity measurements required Hagenbach-Couette correction time (tg 37.31s). Immediately after 2, 5, and 10 times microporations, 97.1432.39, 80.5715.51, and 82.3916.49m sizes of micropores were formed on the skin, respectively. Dextran; Weissella cibaria; exopolysaccharide; prebiotic; skimmed milk. Nair SS. The values of Mark-Houwink parameters could be universalized with certain precautions, being an indication for the calculation of molecular weight in the temperature range of 20-40C. The hydrodynamic radius of 2-NBDG glucose was too small to measure. The Mark-Houwink parameters indicate the dependence with temperature (T) in the range from 20 to 50C, ie with increasing T a increases and kM-H decreases. Molecular weight, molecular weight distribution and molecular size of a native dextran. Transdermal drug delivery is an attractive alternative that addresses the limitations of oral and parenteral routes of drug administration; it enables controlled release and long-term systemic drug delivery through the skin, avoids hepatic first-pass effect, avoids gastrointestinal drug degradation, reduces discomfort and trauma resulting from hypodermic injections, and prevents safety hazards associated with dangerous medical waste from needles [1, 2]. As intrinsic viscosity of dextrans decreases with an increase in solution temperature; chain flexibility enhances with an increase in temperature (see Table 4) showing that the intrinsic viscosity of dextran (with different molecular weights Mw from 8.8 to 200kDa) decreases linearly with an temperature increase in solution, but change to high molecular weight to hyperbranched macromolecule. The relation between Mw and the intrinsic viscosity is given by Mark-Houwink equation. In the case of a poor solvent, a temperature increase causes an increase in entropy and intrinsic viscosity and is favorable for the extended conformation. Karmarkar, S.; Garber, R.: Kluza, J.; Koberda, M. (2006). Colloid and Polymer Science, 286, 1223-1231. 2006;3(3):37385. ; Picout, D.R. Analytes and stationary phase with Universal Calibration Relative Measurement Concentration and MW detector: RI + Viscometer Relationship between hydrodynamic volume (MW*intrinsic viscosity) and elution volume Multiple detection The measurements of the transepidermal water loss (TEWL) were taken using the Tewameter (Courage-Khazaka Electronic GmbH, Cologne, Germany) to evaluate the skin barrier function after microporation. The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was approximately, 800 kDa. Thus, typically, the dextran glomerular for a molecule with a Stokes-Einstein (SE) radius of 30 is approximately sevenfold higher than that of neutral horseradish The qM-H values given by equation 13, these vary from 0.9993 to 0.9841 for the range between 8.8 to 200kDa in a temperature range of 20 to 40C. This device can generate microchannels from the SC through to the outer dermis and allow transdermal delivery of active agents. Polysaccharide hydrogels for modified release formulations. (2008). Dulbeccos Phosphate-Buffered Saline (DPBS) was purchased from Welgene (Gyeongsan, Korea). Data File Dextran. sharing sensitive information, make sure youre on a federal Kasaai, Mohammad R. (2012). Typically, the hydrodynamic diameter of the micelles is about 100 nm and the critical micelle concentration is about 10 mg/L. In vitro studies with human cadaver skin is a viable, ethical, and economical alternative for in vivo animal studies. Ortega, A.; Garca de la Torre, J. International Journal of Biological Macromolecules, 23, 135-141. 2003;89(2):31120. Red blood cell rouleaux formation in dextran solution: dependence on polymer conformation. These structural differences can significantly affect FITC-dextran delivery due to even after microporation [30]. DLS tests were conducted at detection angle of 90 (standard) and 173 (backscatter) to measure protein hydrodynamic radius, while protein charge was examined in phase analysis light scattering (PALS) mode at detection angle of 15 (forward). PMC Equivalent Radii and Ratios of Radii from Solution Properties as Indicators of Macromolecular Conformation, Shape, and Flexibility. Mark-Houwink values confirm that for these conditions dextrans behave as rigid spheres with a tendency at compaction as temperature increases. https://doi.org/10.1186/s40824-020-00201-7, DOI: https://doi.org/10.1186/s40824-020-00201-7. Careers. All branched polysaccharide types could be described approximately by the hyperbranching theory. Markierte Dextrane sind hydrophile Polysaccharide, die hufig in mikroskopischen Studien zur berwachung der Zellteilung, zum Verfolgen der Bewegung von lebenden Zellen und zur Untersuchung der hydrodynamischen Eigenschaften der zytoplasmatischen Matrix verwendet werden. 4h after microporation, the sizes of the micropore decreased slightly. The most widely applied coatings for FDA-approved SPIONs are dextran and carboxydextran. statement and Transdermal delivery is of great importance for the effective delivery of bioactive or therapeutic agents into a body. GYA primarily conducted research. The intrinsic viscosity classical theory of random coils at conditions []0 predicts the quotient a0 = d ln[]0/d ln M to decrease from unity to 0.5 as the chain length increases. The human and pig skin samples were placed in the receiver chamber of the Transdermal diffusion cell system with the SC facing up and the donor chamber was fixed in place. Guner, A.; Kibarer, G. (2001). (1999). Its Invariancy with Respect to the Cross-Sectional Dimensions of Polymer Chains. The skin culture medium was purchased from Biopredic International (Saint-Grgoire, France). Webwhittier union high school district superintendent. The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was approximately, 800 kDa. Home > 2022 > Junho > 22 > Uncategorized > dextran hydrodynamic radius. Molecular weight, particle size and diffusion coefficient can be determined in many studies by Dynamic Light Scattering [20, 21, 22], Gel Permeation Chromatography [23], Analytical Ultracentrifuge [24, intrinsic viscosity [1, 7, 14], size exclusion chromatography / multiangle laser light scattering / differential viscometry, Flow Field-Flow Fractionation [25], and so on.